| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Holdemania filiformis | TGGACGATCATGCTGCTGA | GGACAGATCCAGAGCGCTT | 59.10 | 59.48 | 168 | 63 |
100.00%
|
50.00%
|
| Primer 502 | Holdemania filiformis | GTTCAACCTGCTGACCGGA | TCTTCCAGCACGCGTTGT | 59.93 | 59.89 | 270 | 63 |
100.00%
|
50.00%
|
| Primer 503 | Holdemania filiformis | ACATGCCGATCATCGCGA | TGCGGTCAGAAAGCAGCT | 59.58 | 59.57 | 225 | 63 |
100.00%
|
50.00%
|
| Primer 504 | Holdemania filiformis | CGATGAAACGACGATCGGC | ACTCATGCTTGTCCTCCGC | 59.45 | 60.08 | 194 | 63 |
100.00%
|
50.00%
|
| Primer 505 | Holdemania filiformis | GCGATGAAACGACGATCGG | ACTCATGCTTGTCCTCCGC | 59.45 | 60.08 | 195 | 63 |
100.00%
|
50.00%
|
| Primer 506 | Holdemania filiformis | TCAAACAGCTGCGGACGA | AAACTTCGCCAGCGTCGT | 59.58 | 60.28 | 164 | 63 |
100.00%
|
50.00%
|
| Primer 507 | Holdemania filiformis | ATCAAACAGCTGCGGACGA | AAACTTCGCCAGCGTCGT | 60.01 | 60.28 | 165 | 63 |
100.00%
|
50.00%
|
| Primer 508 | Holdemania filiformis | ACCAGCAGCAACGCGAT | TGCCGACCGAATTGCAGA | 60.01 | 59.65 | 268 | 63 |
100.00%
|
50.00%
|
| Primer 509 | Holdemania filiformis | GACGGCATCATCGGGACTT | ACGGCAGTGTGAGCTTGT | 59.86 | 59.49 | 178 | 63 |
100.00%
|
50.00%
|
| Primer 510 | Holdemania filiformis | AGAAGACGGCTCCTTCGGA | ACGGCAGTGTGAGCTTGT | 60.30 | 59.49 | 251 | 63 |
100.00%
|
50.00%
|